| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Dreamers FC DFC MPA NPL 05G |
03134-0011G6U-1753 | Kenan Sahuric |
| A2 | Gretna Elite Academy GEA ECNL-RL 05G |
02077-0011G6U-0048 | Kyle Marek |
| A3 | Sporting Nebraska FC SOFC G PREMIER 05 |
03036-001PRG6U-2203 | Dennis Franks |
| A4 | Villarreal Nebraska Academy VNA 2005 Ultra Girls |
03131-0011G6U-0001 | Jorge Sambataro |
| B1 | Omaha United Soccer Club Omaha United SC 2005G Black |
03133-0012G6U-0002 | Jose Vargas |
| B2 | Elkhorn Athletic Association NE Roots 2005G I |
03004-0011G6U-1065 | Haggai Leboo |
| B3 | Dreamers FC DFC MPA MWC 2005G |
03134-0011G6U-1752 | David Hawkins |
| B4 | Papillion Soccer Club PSC 2005G Mystics |
02016-0011G6U-0003 | Dennis Miller |