| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Sporting Nebraska FC SOFC B ELITE 05 |
03036-001HEB6U-2102 | Ryan Kruse |
| A2 | Dreamers FC DFC MPA NPL 05B |
03134-0011B6U-1768 | David Hawkins |
| A3 | Gretna Elite Academy GEA NPL 05B |
02077-0011B6U-0041 | Michael Auld |
| A4 | Dreamers FC DFC Aztecs 05 |
03134-0011B6U-0066 | Giovanni Tapia |
| B1 | Nebraska FC NFC Blue 2005 |
03062-0011B6U-0133 | Byron Dacy |
| B2 | z-South Sioux City Soccer Club SSC Diablos Gold 05 - B16U |
04056-0011B6U-0013 | Andy Reyes |
| B3 | Dreamers FC DFC MPA BLUE LINCOLN 05B |
03134-0011B6U-1770 | Kenan Sahuric |
| B4 | Dreamers FC DFC MPA BLUE OMAHA 05B |
03134-0011B6U-1769 | David Hawkins |